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RNAi Core Facility
The RNAi Core Facility provides easy and economical access to the latest in whole genome RNAi collections through the Open Access Program from Open Biosystems. Individual constructs are available as either bacterial stocks or lentivirus. The program currently includes the following libraries: Human and Mouse GIPZ lentiviral shRNAmir Human and Mouse TRC lentiviral shRNA Human TRIPZ inducible shRNAmir RNAi Validation of Knockdowns BMGC will design and validate Roche UPL primer-probe set for gene-of-interest. We will perform RT, Q-RT-PCR for all total RNA samples and provide a validated knockdown report with all raw data for $50 per gene. Validated primer-probe sets can be used for further Q-RT-PCR work at a standard rate of $1 per data point based on technical duplicates. Please contact qpcr@umn.edu.
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