Automated Plasmid Preparation

SUMMARY OF SERVICE

Plasmid Preparation
BMGC has several robotic devices to assist with high-throughput assays.  The Qiagen BioRobot 8000 is configured to process plasmids from the culture stage to that of a sequence-ready template.  This fully automated system can process one 96-well plate per hour, with 4 plates per load, allowing hands-off preparation. 

PRICES

Plasmid Preparation

96-well block plasmid DNA extraction - University CUFS number

• $125 for 96-well block plasmid DNA extraction

• $25 for preparation of 96-well plate for sequencing

96-well block plasmid DNA extraction - Affiliated company

• $150 for 96-well block plasmid DNA extraction

• $25 for preparation of 96-well plate for sequencing

96-well block plasmid DNA extraction - External user

• $200 for 96-well block plasmid DNA extraction

• $35 for preparation of 96-well plate for sequencing

HOW TO ORDER

What needs to be done to receive this service?

96-well blocks can be dropped off or sent on ice to the address listed below.  If automated DNA sequencing is to be done on the samples, please include primer with a concentration of 3.2 pmole/uL. 

To place an online order go to https://www.ahcs.umn.edu/bmgc/index.cfm

What paperwork needs to be completed?

• print Plasmid Preparation Request Form after online order has been submitted

Where can samples be dropped-off?

• St. Paul campus: 20 Snyder Hall - see a staff member when dropping off blocks - Map

Sending samples – shipping address (use ice packs do not use dry ice)

• 1475 Gortner Avenue, Room 20 Snyder Hall, St. Paul, MN  55108

Where can results be obtained?

Extracted DNA samples are stored in 96-well plates at -20 degrees Celsius on St. Paul campus 20 Snyder Hall.  Samples can be picked up during business hours.

What is the turnaround time?

We have a 72-hour turnaround time.  If you have not received an email message before the 72-hour turnaround time, please contact any staff member at 612-625-7736.