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Services
Click on any of the following services to obtain detailed information regarding that service. Information includes pricing and details on how and where to submit samples.
Sanger Sequencing
The BMGC sequencing and genotyping facility utilizes capillary electrophoretic DNA analyzers for quick turnaround on small and high-throughput projects. Three 16 capillary ABI 3130xl and one 96 capillary ABI 3730xl use ABI BigDye version 3.1 Terminator chemistry.
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For information regarding sequencing instrumentation used in our laboratory, see Applied Biosystems website
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For recommended plasmid purification, see Qiagen's website
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For recommended PCR purification, see Millipore's website or Qiagen's website
Primers may be ordered through the Oligonucleotide and Peptide Synthesis Facility. For information regarding primers, go to Integrated DNA Technologies website
Next-Generation Sequencing
BMGC has the GS FLX System, an ultra-high-throughput, next-generation sequencing platform capable of generating up to 400,000 long reads with exceptional accuracy in a single run. For more information regarding this technology, click here. Please contact Sushmita Singh at 612-624-3014 for questions or email regarding custom projects.
GeneScan® or
GeneMapper
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An ABI 3130xl capillary instrument is used for fragment analysis/genotyping using GeneScan® Software.
Plasmid Preparation
BMGC has several robotic devices to assist with high-throughput assays. The Qiagen BioRobot 8000 is configured to process plasmids from the culture stage to that of a sequence-ready template. This fully automated system can process one 96-well plate per hour, with 4 plates per load, allowing hands-off preparation.
Agilent Bioanalyzer 2100
The BMGC has an Agilent Bioanalyzer 2100 available for analysis of DNA, RNA, proteins, and cells. DNA and RNA chips are available for purchase through the lab.
Real Time - PCR
An ABI 7900HT is available to researchers for their real-time PCR needs. The 7900HT has a wavelength detection of 500-660 nm, allowing for multiple chemistry detection, including TAQMAN® and SYBR® Green. Applications include real-time PCR, SNP detection and +/- assays. The 7900HT is equipped with robotic capabilities for multiple plate scanning.
- For information regarding real time PCR instrumentation used in our laboratory, see Applied Biosystems website
Hydra pipetting stations
The BMGC has two 96-well Robbins Scientific Hydra pipetting stations.
- For information regarding the Hydra pipetting station located in our laboratory, click here
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