Genotyping Analysis Service
SUMMARY OF SERVICE
GeneScan® or GeneMapper®
An ABI 3130xl capillary instrument is used for fragment separation. There are 4 different dye sets available:
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DYE SET
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SIZE STANDARD DYE
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6-FAM™, HEX™, NED™
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MapMarker® or ROX™
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6-FAM™, VIC®, NED™
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ROX™
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5-FAM™, JOE™, NED™
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ROX™
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6-FAM™, VIC®, NED™, PET™
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LIZ®
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The size standards available are ROX™-400HD, ROX™-500, LIZ®-500, and MapMarker®-1000.
ROX™-400HD has the following sizes: 50, 60, 90, 100, 120, 150, 160, 180, 190, 200, 220, 240, 260, 280, 290, 300, 320, 340, 360, 380, and 400.
ROX™-500 has the following sizes: 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500.
LIZ®-500 has the following sizes: 35, 50, 75, 100, 139, 150, 160, 200, 250, 300, 340, 350, 400, 450, 490, and 500.
MapMarker®-1000 has the following sizes: 50, 75, 100, 125, 150, 200, 250, 300, 350, 400, 450, 475, 500, 550, 600, 650, 700, 750, 800, 850, 900, 950, and 1000.
PRICES
Plate of 16 or muliples of 16 samples - University CUFS number, Affiliated company purchase order and External user purchase order
HOW TO ORDER
What needs to be done to receive this service?
ABI recommends pooling different dyes after your PCR reaction. Recommended volume per well is 10 uL. For new projects, 16 test dilutions (wells A1-H2) will be run for free for you to optimize the concentration. Prepare and submit samples in multiples of 16 in a 96 well plate with appropriate concentrations and the Fragment Analysis Request Form at a location listed below. Be sure to cover plate securely. If dropping off samples, please cover plate with plate tape. If shipping samples, please cover plate with cap strips.
To place an online order go to https://www.ahcs.umn.edu/bmgc/index.cfm.
What paperwork needs to be completed?
Where can samples be dropped-off?
We have 2 drop-off locations
Sending samples – mailing address
Sending samples – shipping address
Where can results be obtained?
Results are in two different formats: sample files (.fsa) and exported tables (Excel files). There are a few programs to view .fsa files: GeneScan®, GeneProfiler, GeneMapper®, PeakScanner™. Excel files are only available for files analyzed in GeneScan® or GeneProfiler.
Placement of results: email, BMGC Data Management System, FTP SITE, or HARD DRIVE
What is the turnaround time?
We have a 72-hour turnaround time. If you have not received an email message before the 72-hour turnaround time, please contact any staff member at 612-625-7736.
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