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Services
Oligonucleotide Synthesis
The Oligonucleotide & Peptide Synthesis Facility offers High quality DNA both locally synthesized and by IDT. There is also local 24 hr service for primers (limited #). IDT offers all the possible modifications and Labels. University of Minnesota researchers ordering Offsite Nucleotides via the Oligonucleotide & Peptide Synthesis Facility get better pricing and the convenience of using CUFS.
Resources – (link to view a table of our synthesis equipment)
Ordering/Submitting
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Please submit the Excel Custom Oligo Order Form as an attachment to: Oligo@lenti.med.umn.edu
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For further information call 612-624-3177
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For pick up we will either call you or e-mail when the primers are ready.
Peptide Synthesis
The Oligonucleotide & Peptide Synthesis Facility, as part of the BioMedical Genomics Center, provides flexible, high quality, Peptides. To ensure quality in our service we aim to work closely with investigators. After an initial discussion about desired peptide, Synthesis and/or purification will be carried out by our experienced staff.
Resources- (link to view a table of our synthesis equipment)
Ordering/Submitting
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Please drop off your completed Peptide Order Form to 1-202 BSBE (Map) or e-mail the order to walek001@umn.edu
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Please allow about 2 weeks for a crude peptide and 3-4 weeks for a purified peptide to be completed
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For further information call 612-624-3177
N-terminal Protein Sequencing
N-terminal protein sequence information continues to play a significant role in modern structural and molecular biology. It can be used to: aid the identification of unknown proteins; provide data for the design of oligonucleotide/PCR primers; confirm recombinant protein identity and fidelity (reading frame, translation start point, etc.); aid the interpretation of NMR and crystallographic data; demonstrate degrees of identity between proteins; or provide data for the design of synthetic peptides for antibody generation, etc.
N-terminal sequencing utilizes the well-established Edman degradative chemistry, sequentially removing amino acid residues from the N-terminus of the protein and identifying them by reverse-phase HPLC. Sensitivity is at the level of 100s femtomoles and long sequence reads (20-40 residues) can often be obtained from a few 10s picomoles of starting material. Pure proteins (>90%) generate easily interpreted data, but insufficiently purified protein mixtures may also provide useful data, subject to rigorous data interpretation!
N-terminally modified (especially acetylated) proteins cannot be sequenced directly, as the absence of a free primary amino-group prevents the Edman chemistry. However, limited proteolysis of the blocked protein (e.g. using cyanogen bromide) may allow a mixture of amino acids to be generated in each cycle of the instrument, which can be subjected to database analysis in order to interpret meaningful sequence information.
Resources- (link to view a table of our synthesis equipment)
Ordering/Submitting
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