Oligonucleotide and Peptide Synthesis Facility

What's Inside Oligonucleotide and Peptide Synthesis Facility
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The Oligonucleotide & Peptide Synthesis Facility employs state of the art equipment, including ABI/7500 fast RT-PCR, ABI/Pioneer and 433A Peptide Synthesizers, ABI394 and Beckmann 1000 Oligo Nucleotide synthesizers, MALDI for QC and several Analytical and Preparatory HPLC systems for Purifications and QC. The facility also offers off site (IDT) Synthesis of Nucleotides.

SUMMARY OF SERVICES PROVIDED BY THE FACILITY INCLUDE

Oligonucleotide Synthesis
The Oligonucleotide & Peptide Synthesis Facility offers High quality DNA both locally synthesized and by IDT.  There is also local 24 hr service for primers (limited #). IDT offers all the possible modifications and Labels.  University of Minnesota researchers ordering Offsite Nucleotides via the Oligonucleotide & Peptide Synthesis Facility get better pricing and the convenience of using CUFS.

Real-Time PCR
An ABI7500 Fast is available to researchers for their real-time PCR needs.  The 7500 Fast has a wavelength detection of 500-670 nm, allowing for multiple chemistry detection, including TAQMAN® and SYBR® Green.  Applications include real-time PCR, SNP detection and +/- assays, Allelic Discrimination, Relative & Absolute Quantification. 

Peptide Synthesis
The Oligonucleotide & Peptide Synthesis Facility, as part of the BioMedical Genomics Center, provides flexible, high quality, Peptides. To ensure quality in our service we aim to work closely with investigators. After an initial discussion about desired peptide, Synthesis and/or purification will be carried out by our experienced staff.

N-Terminal Protein Sequencing
N-terminal protein sequence information continues to play a significant role in modern structural and molecular biology.  It can be used to: aid the identification of unknown proteins; provide data for the design of oligonucleotide/PCR primers; confirm recombinant protein identity and fidelity (reading frame, translation start point, etc.); aid the interpretation of  NMR and crystallographic data; demonstrate degrees of identity between proteins; or provide data for the design of synthetic peptides for antibody generation, etc.