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Services
Affymetrix Genechip Analysis
We currently have one Genechip 3000 scanner capable of scanning the most recent high density Affymetrix arrays and 2 fluidics stations. We are able to process up to 24 samples per day. Researchers are responsible for providing biotin labeled cRNA that has been processed per Affymetrix standard protocols. Recommended reagents and supplies are noted in the Genechip Technical Expression Manual.
Please fill out an Expression Analysis Request form completely and bring this form with your samples to 1-246 Hasselmo Hall. Samples are processed on a first come, first serve basis.
Qiagen Target Prep Robot
The Microarray Facility has online and operational a Qiagen Target prep robot for synthesizing and biotin labeling total RNA samples for Affymetrix Gene Chip arrays. This instrument is able to process up to 96 samples per run. Researchers must provide 5ug total RNA in 9ul H2O. Cost per sample is $265. Please contact lab personnel for any questions regarding this instrument.
Custom cDNA/Oligo Array Services
Printing Custom Arrays:
The facility has available to researchers 2 Biorobotics Microgrid II spotters for creating custom arrays. This instrument is capable of printing up to 22,000 spots on a typical glass slide. Experienced staff members are available to assist researchers in planning array layouts and making appropriate decisions regarding hardware and probe creation issues. A Scanarray 5000 scanner capable of visualizing many commonly utilized fluorophores is available in BIPL and at the St. Paul campus to create images of hybridized samples for downstream analysis. To reserve the Scanarray 5000 scanners at either campus online, please visit the following link, http://bipl1.med.umn.edu/select_resource.php?v=1.
Please Note: The Microarray facility will now be charging UMN investigators for use of scanarray machines on both Minneapolis and St. Paul campuses beginning April 1st, 2008. The charge will be $30 per hour in increments of $15 every half hour (for example, if you use the machine for 1h and 15min or 1h and 25min, your charge will be $45) .
Please provide /add a valid CUFs number to your existing/new account when you sign up online to reserve time. This measure is being taken to cover charges for repair and maintenance of the equipment.
We apologize for any inconvenience this may cause you. Please contact Archana Deshpande at 612-624-0437 or deshp008@umn.edu if you have any questions.
Please contact Archana Deshpande at 612-624-0437 or email deshp008@umn.edu, to arrange for custom microarrays. The charges for spotting are $45/hour for University of Minnesota researchers, there is no labor charge. For Non-University researchers, the charges are $100/hour for instrument time and $125/hour labor time. These prices are effective from March 1, 2007.
Printing BMAP and NIA chips:
We print mouse BMAP and NIA chips on poly-l-lysine coated slides. The slides are made to order so an advanced notice of at least 3 weeks is advisable. The arrays are printed in batches of 50 arrays and are priced at $100/array. For more information on these arrays, protocols and batches printed please visit the following link. http://www.ahc.umn.edu/genomics_files/MainPage.htm
For ordering information on the BMAP and NIA arrays, please contact Archana Deshpande at 612-624-0437 or email deshp008@umn.edu
Hybridization and Scanning of Microarray chips:
We are now offering hybridization and scanning services for custom microarray users. We have the MAUI 12-bay hybridization system with airbladders that inflate and deflate to move the hybridization solution to increase sensitivity and reproducibility. Researchers will need to bring their post-processed slides and labeled samples and we can perform the hybridization, post-hyb washing and scanning for you and provide the data on CDs in 'tiff' or 'bitmap' file format. However you can opt to do your own scanning and the prices for the services provided are itemized for your convenience.
Hybridization Pricing:
For University of Minnesota researchers: Total $43 to $57 per slide (price varies depending on the mixer used). The total includes the price of the mixer (from $31 to $45) plus $12 for reagents, consumables and labor per slide.
For Non-University of Minnesota researchers: $85 per slide per sample.
Scanning Pricing:
For University of Minnesota researchers: Total $15 per slide. We have a HP Scanarray 5000 with Scanarray express software that can scan up to five different fluors on one side.
For Non-University of Minnesota researchers: $40 per slide.
Please contact Archana Deshpande at 612-624-0437 or email deshp008@umn.edu, to avail of our custom hybridization and scanning services.
Agilent 2100 Bioanalyzer
An Agilent Bioanalyzer is available in 1-246 Hasselmo Hall. The Bioanalyzer is a microchip platform that allows quality and quantity analysis of very small amounts of RNA samples prior to labeling in a microarray experiment. It is highly recommended that this be done to not waste time and money generating samples from poor quality RNA. Chips and reagents are available through the Microarray Facility at a cost of $30.00/Chip for University of Minnesota Researchers. For Non-University researchers the charge is $40.00/Chip. Researchers are asked to provide their own RNA ladder for quantity assessment. There is a one time training fee of $60 for first time users who want to get trained.
NanoDrop ND-1000 Spectrophotometer
The NanoDrop ND-1000 is a full-spectrum (220-750nm) spectrophotometer that measures 1ul samples with high accuracy and reproducibility. It untilizes a patented sample retention technology that employs surface tension alone to hold the sample in place eliminating the need for expensive cuvettes and other devices and allows for clean up in seconds. The ND-1000 also has the capability to measure highly concentrated samples without dilution (50X higher concentration compared to standard specs). There is a $2.50 charge on every visit for up to 10 samples. For more than 10 samples there is a 50 cent charge on each additional sample.
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