Academic Health Center

University of Minnesota Genomics Center

Core Facility

Sanger

Sanger Sequencing

The UMGC utilizes capillary electrophoretic DNA analyzers for quick turnaround on small and high-throughput projects. The facility currently has one 96 capillary ABI 3730xl that use ABI BigDye Terminator version 3.1 chemistry.


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NEW SANGER SERVICE OPTIONS
  1. Sanger Classic (Individual)
  2. Sanger High Throughput (Plates)
  3. Sanger Sample QC. Template and Primer Quantified and Normalized. Template and Primer Addition.
  4. Clean & Load
  5. Sanger Custom
  6. Difficult Template Panel
 
QUALITY SCORES FOR CLIENTS

Quality Values: Calculation and display of quality values (QVs) for pure and mixed bases. The QV is a per-base estimate of the basecaller accuracy.

Sample Score: Calculation and display of a sample score is generated from QVs. It is the average quality value of the bases in the clear range sequence for that sample.

Signal/Noise Value: Calculation and display of the value that represents the average signal/noise value of all the bases in a sample.

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  • Last modified on June 28, 2012