Sanger Sequencing
The ABI PRISM™ 3730xl DNA Analyzer is a fully automated, 96-capillary electrophoresis instrument designed for use in production-scale DNA analysis.
The ABI PRISM® BigDye® Terminator version 3.1 Cycle Sequencing Kit is ideal for finishing with PCR product, plasmid, fosmid, and BAC templates.
Sanger Sequencing
The UMGC utilizes capillary electrophoretic DNA analyzers for quick turnaround on small and high-throughput projects. The facility currently has one 96 capillary ABI 3730xl that use ABI BigDye Terminator version 3.1 chemistry.
NEW SANGER SERVICE OPTIONS
- Sanger Classic (Individual)
- Sanger High Throughput (Plates)
- Sanger QC
- Clean & Load
- Sanger Custom
- Difficult Template Panel
QUALITY SCORES FOR CLIENTS
Quality Values: Calculation and display of quality values (QVs) for pure and mixed bases. The QV is a per-base estimate of the basecaller accuracy.
Sample Score: Calculation and display of a sample score is generated from QVs. It is the average quality value of the bases in the clear range sequence for that sample.
Signal/Noise Value: Calculation and display of the value that represents the average signal/noise value of all the bases in a sample.