The UMGC utilizes capillary electrophoretic DNA analyzers for quick turnaround on small and high-throughput projects. The facility currently has one 96 capillary ABI 3730xl that use ABI BigDye Terminator version 3.1 chemistry.
NEW SANGER SERVICE OPTIONS
- Sanger Classic (Individual)
- Sanger High Throughput (Plates)
- Sanger Sample QC. Template and Primer Quantified and Normalized. Template and Primer Addition.
- Clean & Load
- Sanger Custom
- Difficult Template Panel
QUALITY SCORES FOR CLIENTS
Quality Values: Calculation and display of quality values (QVs) for pure and mixed bases. The QV is a per-base estimate of the basecaller accuracy.
Sample Score: Calculation and display of a sample score is generated from QVs. It is the average quality value of the bases in the clear range sequence for that sample.
Signal/Noise Value: Calculation and display of the value that represents the average signal/noise value of all the bases in a sample.