Protein Sequencing

Protein sequencing uses an ABI Procise sequencer. N-terminal protein sequencing utilizes the well-established Edman degradation chemistry, sequentially removing amino acid residues from the N-terminus of the protein and identifying them by reverse-phase HPLC. It can: aid the identification of unknown proteins; provide data for the design of oligonucleotide/PCR primers; confirm recombinant protein identity and fidelity (reading frame, translation start point, etc.); aid in the interpretation of NMR and crystallographic data; demonstrate degrees of identity between proteins; or provide data for the design of synthetic peptides for antibody generation.