Academic Health Center

University of Minnesota Genomics Center

Core Facility

Illumina Next-generation

Illumina Next-generation sequencing


HiSeq 2000:

llumina’s HiSeq 2000 is the largest next-generation high-throughput sequencing system. This very flexible system allows single read or paired end reads with lengths of 50 or 100 base pairs. This is the preferred platform for RNA-seq applications and is capable of handling a variety of whole genome applications as mentioned previously. The HiSeq 2000 can be operated in single or dual flow cell mode and its independent operable flow cells allow applications requiring different read lengths to run simultaneously. It also boasts the ability to multiplex several samples per lane for increased sample throughput.  Read More





The Illumina MiSeq is a single-lane cousin to the HiSeq 2000. The MiSeq permits very rapid runs, and has a form factor (one lane per run) suited to pilot runs, low-coverage experiments and some metagenomic applications. It is capable of generating more than 6 Gb of data for each 2 x 250 base pair run. The MiSeq also has the ability to multiplex several samples per run. Currently, the system allows single read with a length of 50 or paired end reads with lengths of 150 or 250 base pairs.   Read More


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  • Last modified on June 28, 2012