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University of Minnesota Genomics Center

Core Facility

Illumina Next-generation

Illumina Next-generation sequencing


HiSeq 2500/2000:

llumina’s HiSeq 2500 configuration allows the use of Illumina's two lane low cells and rapid SBS chemistry. The primary benefits of the 2500 configuration are the ability to cluster on instrument, generate longer reads (2 x 150 bp) with higher sequencing quality in later chemistry cycles with reduced run times. Note that while individual flow cell run times may be reduced, we forecast a limited reduction in project turnaround times using this platform due to lower lane yield and multiplexing capacity. Researchers may choose full lanes or half lanes. Read More

llumina’s HiSeq 2000 is the largest next-generation high-throughput sequencing system. This very flexible system allows single read or paired end reads with lengths of 50 or 100 base pairs. This is the preferred platform for RNA-seq applications and is capable of handling a variety of whole genome applications as mentioned previously. The HiSeq 2000 can be operated in single or dual flow cell mode and its independent operable flow cells allow applications requiring different read lengths to run simultaneously. It also boasts the ability to multiplex several samples per lane for increased sample throughput. Researchers may choose full lanes or half lanes.  Read More





The Illumina MiSeq is a single-lane cousin to the HiSeq 2000. The MiSeq permits very rapid runs, and has a form factor (one lane per run) suited to pilot runs, low-coverage experiments and some metagenomic applications. It is capable of generating more than 6 Gb of data for each 2 x 250 base pair run. The MiSeq also has the ability to multiplex several samples per run. Currently, the system allows single read with a length of 50 or paired end reads with lengths of 150 or 250 base pairs.   Read More


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  • Last modified on June 28, 2012