Sample Quantification and Quality Control

1. Qualitative Analysis by Capillary Electrophoresis: UMGC has a Caliper GX and an Agilent Bioanalyzer 2100 for analysis of DNA, RNA, proteins, and cells.
2. Sample Quantification: UMGC has a NanoDrop 8000 full-spectrum spectrophotometer for quantification of DNA and RNA samples. In addition, we have a BioTek Plate Reader used to perform PicoGreen and RiboGreen fluorescent assays for ultrasensitive quantification of nucleic acids.
    

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UMGC provides easy and economical access to the latest in whole genome RNAi collections through the Open Access Program from Open Biosystems. Individual constructs are available as either bacterial stocks or lentivirus. The program currently includes the following libraries:

 Human and Mouse GIPZ lentiviral shRNAmir
Human and Mouse TRC lentiviral shRNA
Human TRIPZ inducible shRNAmir

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RNAi Validation of Knockdowns

UMGC will design and validate Roche UPL primer-probe set for gene-of-interest. We will perform RT, Q-RT-PCR for all total RNA samples and provide a validated knockdown report with all raw data. Validated primer-probe sets can be used for further Q-RT-PCR work. Please contact qpcr@umn.edu for more information.

  

UMGC offers custom peptide synthesis services in quantities ranging from 1 mg to grams. Peptides can be ordered as crude, purified, or on resin. Modifications such as acetylation, biotinylation, di-sulfide bridges, etc. are also available. Peptide QC includes mass spec analysis and determination of peptide purity by RP-HPLC. Peptides are quoted individually and pricing is dependent on sequence, level of purity, etc.

 

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UMGC offers custom DNA, RNA, Ultramers and 96 and 384 plate Oligo synthesis services in quantities ranging from 10 nmole to 10umole scales through IDT. Oligos can be ordered as individual tubes or in plate format as well as standard desalted or purified (HPLC or PAGE). IDT offers a wide range of Modifications. Oligo QC includes individual mass spec analysis and CE reports.

 

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UMGC provides DNA Extraction with IRB Approval from Individual blood samples from Vacutainer or 96 well plate format as well as Oragene (tube or disc format).

We also provide custom DNA and RNA from Tissue, FFPE, frozen pellets, etc.

We will archive your samples or ship them to a specific location.

UMGC provides a variety of additional services for extracted samples. They range from quality control, normalizing, sample pulling and plating, etc.

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Items from ThermoFisher can be ordered through our online ordering system.