Service Options
RNAi Service Options
Individual Bacterial Clones
We provide access to our GIPZ and TRC lentiviral shRNA libraries in the form of bacterial agar stabs. This simple form allows for maximum savings and quick access so you can start your experiment immediately.
Economical Price: Savings of 95% when compared to retail pricing through Open Biosystems
Fast Service: Orders are processed same day or next day (Monday-Thursday) for pick-up availability in 1-2 business days.
The TRIPZ library is not available on-site at the RNAi Core Facility, but we have special pricing available for our researchers. TRIPZ clones are ordered using the same method as GIPZ and TRC, but we receive the TRIPZ clones once weekly directly from Open Biosystems.
To search your gene of interest and order individual clones, log into the Online Database.
More Information
Library Information
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GIPZ lentiviral shRNAmir
Developed by Open Biosystems, the GIPZ library utilizes an shRNAmir hairpin that is expressed as microRNA transcripts. This design allows for increased processing and greater knockdown when compared to normal shRNA hairpins (Silva et al 2005). The GIPZ library is available on-site and currently consists of 71,000 human clones and 57,000 mouse clones. The overall goal of Open Biosystems is to expand the current human library to 150,000 clones with 5-6 per gene target.
Vector Highlights- shRNAmir design for specificity and increased knockdown
- GFP marker visualizes hairpin expression
- Efficient low copy knockdown, important for pooled screens
- Perform efficient RNAi in primary and non-dividing cells
- Create stable cell lines with puromycin selection
- Genome-wide human and mouse coverage
- 1-3 clones per gene
Deatiled vector map and sequence (pdf)
Vector Element Utility CMV Promoter RNA polymerase II promoter cPPT Central polypurine tract helps translocation into the nucleus of non-dividing cells WRE Enhances the stability and translation of transcripts turboGFP Marker to track shRNAmir expression Puro(r) Mammalian selectable marker Amp(r) Ampicillin bacterial selectable marker 5'LTR 5' long terminal repeat pUC ori High copy replication and maintenance in E. coli SIN-LTR 3' Self-inactivating long terminal repeat RRE Rev response element Zeo(r) Zeocin bacterial selectable marker -
TRC lentiviral shRNA
The RNAi Consortium was founded at the Broad Institute as a collaboration between MIT and Harvard. The TRC library was created to target about 15,000 human and 15,000 mouse genes with 5-6 targets per gene. The TRC library is available on-site and consists of 85,000 human clones and 80,000 mouse clones.
Vector Highlights
- Rules-based shRNA design for efficient knockdown
- Perform efficient RNAi in primary and non-dividing cells
- Create stable cell lines with puromycin selection
- 4-5 clones per gene target allow for broader coverage
Detailed vector map and sequence (pdf)
Vector Element Utility Human U6 Promoter RNA generated with four uridine overhangs at each 3' end PGK phosphoglycerate kinase promoter Puro(r) Puromycin mammalian selectable marker SIN LTR 3' Self-inactivating long terminal repeat f1 ori f1 origin of replication AMP(r) Ampicillin bacterial selectable marker 5' LTR 5' long terminal repeat RRE Rev response element -
TRIPZ inducible shRNAmir
The TRIPZ system, developed by Open Biosystems, uses the shRNAmir hairpin design in conjunction with an inducible Tet-On promoter. This allows for creation of a stable cell line without hairpin expression or gene knockdown. The RNAi Core Facility does not have the TRIPZ library on-site, but the library clones are searchable in our database and ordering is available at special pricing. Please see the service option for more information.
- Tet-inducible knockdown
- shRNAmir design for specificity and increased knockdown
- RFP marker visualizes hairpin expression
- Perform efficient RNAi in primary and non-dividing cells
- Create stable cell lines with puromycin selection
- Genome-wide human coverage
Detailed vector map and sequence (pdf)
Vector Element Utility TRE Tet-inducible promoter rt TA3 Reverse tet-transactivator UBC promoter Drives rtTA3 expression of rtTA3 and IRES-puro cPPT Central polypurine tract helps translocation into the nucleus of non-dividing cells WRE Enhances the stability and translation of transcripts turbo RFP Marker to track inducible shRNAmir expression Puro(r) Mammalian selectable marker Amp(r) Ampicillin bacterial selectable marker 5' LTR 5' long terminal repeat pUC Ori High-copy replication and maintenance in E. coli SIN-LTR 3' Sel-inactivating long terminal repeat RRE Rev response element Zeo(r) Zeocin bacterial selectable marker