Academic Health Center

University of Minnesota Genomics Center

Core Facility

Service Options

RNAi Service Options

Individual Bacterial Clones 

AHCBMGC2_image_220x140_Stab_01

We provide access to our GIPZ and TRC lentiviral shRNA libraries in the form of bacterial agar stabs. This simple form allows for maximum savings and quick access so you can start your experiment immediately. 

Economical Price: Savings of 95% when compared to retail pricing through Open Biosystems
Fast Service: Orders are processed same day or next day (Monday-Thursday) for pick-up availability in 1-2 business days.

 

AHCBMGC2_image_220x140_Stab_02

The TRIPZ library is not available on-site at the RNAi Core Facility, but we have special pricing available for our researchers.  TRIPZ clones are ordered using the same method as GIPZ and TRC, but we receive the TRIPZ clones once weekly directly from Open Biosystems.

To search your gene of interest and order individual clones, log into the Online Database.

 

 

Library Information
  • GIPZ lentiviral shRNAmir

    Developed by Open Biosystems, the GIPZ library utilizes an shRNAmir hairpin that is expressed as microRNA transcripts.  This design allows for increased processing and greater knockdown when compared to normal shRNA hairpins (Silva et al 2005).  The GIPZ library is available on-site and currently consists of 71,000 human clones and 57,000 mouse clones. The overall goal of Open Biosystems is to expand the current human library to 150,000 clones with 5-6 per gene target.  

     
    Vector Highlights

    • shRNAmir design for specificity and increased knockdown
    • GFP marker visualizes hairpin expression
    • Efficient low copy knockdown, important for pooled screens
    • Perform efficient RNAi in primary and non-dividing cells
    • Create stable cell lines with puromycin selection
    • Genome-wide human and mouse coverage
    • 1-3 clones per gene

    pGIPZ vector simple

    Deatiled vector map and sequence (pdf)

    Vector Element Utility
    CMV Promoter RNA polymerase II promoter
    cPPT Central polypurine tract helps translocation into the nucleus of non-dividing cells
    WRE Enhances the stability and translation of transcripts
    turboGFP Marker to track shRNAmir expression
    Puro(r) Mammalian selectable marker
    Amp(r) Ampicillin bacterial selectable marker
    5'LTR 5' long terminal repeat
    pUC ori High copy replication and maintenance in E. coli
    SIN-LTR 3' Self-inactivating long terminal repeat
    RRE Rev response element
    Zeo(r) Zeocin bacterial selectable marker
  • TRC lentiviral shRNA

    The RNAi Consortium was founded at the Broad Institute as a collaboration between MIT and Harvard.  The TRC library was created to target about 15,000 human and 15,000 mouse genes with 5-6 targets per gene.  The TRC library is available on-site and consists of 85,000 human clones and 80,000 mouse clones.   


    Vector Highlights

    • Rules-based shRNA design for efficient knockdown
    • Perform efficient RNAi in primary and non-dividing cells
    • Create stable cell lines with puromycin selection
    • 4-5 clones per gene target allow for broader coverage

    pLKO.1 vector simple

    Detailed vector map and sequence (pdf)

    Vector Element Utility
    Human U6 Promoter RNA generated with four uridine overhangs at each 3' end
    PGK phosphoglycerate kinase promoter
    Puro(r) Puromycin mammalian selectable marker
    SIN LTR 3' Self-inactivating long terminal repeat
    f1 ori f1 origin of replication
    AMP(r) Ampicillin bacterial selectable marker
    5' LTR 5' long terminal repeat
    RRE Rev response element
  • TRIPZ inducible shRNAmir

    The TRIPZ system, developed by Open Biosystems, uses the shRNAmir hairpin design in conjunction with an inducible Tet-On promoter.  This allows for creation of a stable cell line without hairpin expression or gene knockdown.  The RNAi Core Facility does not have the TRIPZ library on-site, but the library clones are searchable in our database and ordering is available at special pricing.  Please see the service option for more information.        


     Vector Highlights

    • Tet-inducible knockdown 
    • shRNAmir design for specificity and increased knockdown
    • RFP marker visualizes hairpin expression
    • Perform efficient RNAi in primary and non-dividing cells
    • Create stable cell lines with puromycin selection
    • Genome-wide human coverage

    TRIPZ vector simple

    Detailed vector map and sequence (pdf)

    Vector Element Utility
    TRE Tet-inducible promoter
    rt TA3 Reverse tet-transactivator
    UBC promoter Drives rtTA3 expression of rtTA3 and IRES-puro
    cPPT Central polypurine tract helps translocation into the nucleus of non-dividing cells
    WRE Enhances the stability and translation of transcripts
    turbo RFP Marker to track inducible shRNAmir expression
    Puro(r) Mammalian selectable marker
    Amp(r) Ampicillin bacterial selectable marker
    5' LTR 5' long terminal repeat
    pUC Ori High-copy replication and maintenance in E. coli
    SIN-LTR 3' Sel-inactivating long terminal repeat
    RRE Rev response element
    Zeo(r) Zeocin bacterial selectable marker
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  • Last modified on November 5, 2012