BioMedical Genomics Center: Advancing genomics research, education, & outreach From its inception in the spring of 2000, the BioMedical Genomics Center at the University of Minnesota has been advancing genomics research as it applies to human and animal health. The BMGC continues to maintain, upgrade, and acquire state-of-the-art instrumentation for genomics focused research. The BMGC is a leader in the development of genomics-related education and outreach through specialized conferences, seminars, and workshops. Through these efforts, the BMGC has helped establish entrepreneurial pathways linking genomics discoveries to the founding of knowledge-based companies in the state of Minnesota. ____________________________ U of MN/BMGC will be closed for business on the following dates: Monday May 28, 2012 (Memorial Day) Wednesday July 4, 2012 (Independence Day) Monday September 3, 2012 (Labor Day) Thursday, Friday November 22, 23, 2012 (Thanksgiving) Monday, Tuesday December 24, 25 2012 (Christmas) Shortcuts: BMGC Services GENOTYPING OLIGONUCLEOTIDE & PEPTIDE SYNTHESIS MICROARRAY EXPRESSION DNA & RNA EXTRACTION RNAi SANGER SEQUENCING
NEXT-GENERATION SEQUENCING
FRAGMENT ANALYSIS REAL TIME- PCR (Q-RT-PCR EXPRESSION) SAMPLE QUANTIFICATION AND QUALITY CONTROL | | University researchers to place online orders Following request forms currently available. - Oligonucleotide & Peptide Synthesis and Protein Sequencing
- Genotyping, Epigenomics and CNV Analysis
- DNA Sequencing and Analysis
- Expression Analysis
- Sample Quantification and Quality Control
_____________________________ Illumina HiSeq 2000 & Illumina MiSeq The BMGC is happy to announce that in December, we received shipment on a second Illumina HiSeq 2000 instrument. We traded in our (outdated) Illumina GA IIx sequencer for a HiSeq 2000 instrument, the GA IIx will be taken off-line on Jan. 31, 2012. Compared to the GA IIx, the HiSeq 2000 generates 16X more data per run. Now, with the possession of two HiSeq 2000’s, we will be better able to meet demand, and avoid the long backlogs that occurred during 2011. The second HiSeq also provides instrument redundancy, thereby permitting us to continue to function during (inevitable) periods of instrument failure and repair. Note: there are known difference between the two instruments that may influence RNA-Seq study results. If you have any questions or concerns regarding this forthcoming change in service, please contact Aaron Becker becke044@umn.edu or Nichole Peterson peter585@umn.edu or call the BMGC (612) 625-7736. The BMGC also received shipment of our Illumina MiSeq, a single-lane cousin to the HiSeq 2000.
The MiSeq permits very rapid runs, and has a form factor (one lane per run) suited to pilot runs, low-coverage experiments (some metagenomic applications), and long reads (currently, 2x150-bp paired-end reads, with an announcement of lengths of 2 x 250-bp by mid-2012). More information about the MiSeq will be made available in the coming quarter.
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